ELK4847-48T, Human dLL3(Delta Like Protein 3) ELISA Kit, 48T

ELK4847-48T, Human dLL3(Delta Like Protein 3) ELISA Kit, 48T

ELK4848-48T, Human JAM2(Junctional Adhesion Molecule 2) ELISA Kit, 48T

ELK4848-48T, Human JAM2(Junctional Adhesion Molecule 2) ELISA Kit, 48T

ELK4847-96T, Human dLL3(Delta Like Protein 3) ELISA Kit, 96T

2.963,10 RON

Human dLL3(Delta Like Protein 3) ELISA Kit

SKU
ELK4847-96T

Alternative Names: SCDO1; Delta3; Drosophila Delta homolog 3

Species: Human

Assay Type: Sandwich

Sensitivity: 0.069 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Signal transduction;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human dLL3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human dLL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human dLL3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human dLL3 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: SCDO1; Delta3; Drosophila Delta homolog 3

Species: Human

Assay Type: Sandwich

Sensitivity: 0.069 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Signal transduction;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human dLL3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human dLL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human dLL3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human dLL3 in the samples is then determined by comparing the OD of the samples to the standard curve.