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ELK4821-96T, Chicken OC(Osteocalcin) ELISA Kit, 96T
3.736,60 RON
Chicken OC(Osteocalcin) ELISA Kit
SKU
ELK4821-96T
Alternative Names: BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein
Species: Chicken
Assay Type: Sandwich
Sensitivity: 0.67 pg/mL
Standard: 100 pg/mL
Detection range: 1.57-100 pg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Metabolic pathway;Endocrinology;Hormone metabolism;Bone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken OC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken OC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken OC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken OC in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 3.140,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein Species: Chicken Assay Type: Sandwich Sensitivity: 0.67 pg/mL Standard: 100 pg/mL Detection range: 1.57-100 pg/mL Sample type: Serum, plasma and other biological fluids. Assay length: 3.5h Research Area: Metabolic pathway;Endocrinology;Hormone metabolism;Bone metabolism; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken OC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken OC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken OC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken OC in the samples is then determined by comparing the OD of the samples to the standard curve. |
