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ELK4798-96T, Human GOD(Glucose Oxidase) ELISA Kit, 96T
2.963,10 RON
Human GOD(Glucose Oxidase) ELISA Kit
SKU
ELK4798-96T
Alternative Names: Gox; Glucose Oxidase Enzyme
Species: Human
Assay Type: Sandwich
Sensitivity: 0.113 ng/mL
Standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GOD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GOD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GOD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GOD in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Gox; Glucose Oxidase Enzyme Species: Human Assay Type: Sandwich Sensitivity: 0.113 ng/mL Standard: 20 ng/mL Detection range: 0.32-20 ng/mL Sample type: Tissue homogenates, cell lysates and other biological fluids. Assay length: 3.5h Research Area: Enzyme & Kinase;Metabolic pathway; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GOD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GOD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GOD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GOD in the samples is then determined by comparing the OD of the samples to the standard curve. |
