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ELK4723-96T, Mouse OX(Orexin) ELISA Kit, 96T
2.963,10 RON
Mouse OX(Orexin) ELISA Kit
SKU
ELK4723-96T
Alternative Names: HCRT; PPOX; Orexin A; Hypocretin 1; Orexin B; Hypocretin 2; Preproorexin; Hypocretin Neuropeptide Precursor
Species: Mouse
Assay Type: Competitive Inhibition
Sensitivity: 2.65 pg/mL
Standard: 500 pg/mL
Detection range: 7.82-500 pg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2h
Research Area: Neuro science;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse OX protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse OX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse OX in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: HCRT; PPOX; Orexin A; Hypocretin 1; Orexin B; Hypocretin 2; Preproorexin; Hypocretin Neuropeptide Precursor Species: Mouse Assay Type: Competitive Inhibition Sensitivity: 2.65 pg/mL Standard: 500 pg/mL Detection range: 7.82-500 pg/mL Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 2h Research Area: Neuro science; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse OX protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse OX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse OX in the samples is then determined by comparing the OD of the samples to the standard curve. |
