ELK4658-48T, Human ARPC2(Actin Related Protein 2/3 Complex Subunit 2) ELISA Kit, 48T

ELK4658-48T, Human ARPC2(Actin Related Protein 2/3 Complex Subunit 2) ELISA Kit, 48T

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ELK4658-96T, Human ARPC2(Actin Related Protein 2/3 Complex Subunit 2) ELISA Kit, 96T

2.963,10 RON

Human ARPC2(Actin Related Protein 2/3 Complex Subunit 2) ELISA Kit

SKU
ELK4658-96T

Alternative Names: ARC34; PNAS-139; p34-Arc; Arp2/3 complex 34 kDa subunit

Species: Human

Assay Type: Sandwich

Sensitivity: 0.068 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Cardiovascular biology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ARPC2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ARPC2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ARPC2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ARPC2 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: ARC34; PNAS-139; p34-Arc; Arp2/3 complex 34 kDa subunit

Species: Human

Assay Type: Sandwich

Sensitivity: 0.068 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Cardiovascular biology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ARPC2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ARPC2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ARPC2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ARPC2 in the samples is then determined by comparing the OD of the samples to the standard curve.