ELK4648-48T, Human RHO(Rhodopsin) ELISA Kit, 48T

ELK4648-48T, Human RHO(Rhodopsin) ELISA Kit, 48T

ELK4649-48T, Human PTGIR(Prostacyclin Receptor) ELISA Kit, 48T

ELK4649-48T, Human PTGIR(Prostacyclin Receptor) ELISA Kit, 48T

ELK4648-96T, Human RHO(Rhodopsin) ELISA Kit, 96T

3.012,90 RON

Human RHO(Rhodopsin) ELISA Kit

SKU
ELK4648-96T

Alternative Names: OPN2; RP4; Opsin 2; Rod Pigment; Retinitis Pigmentosa 4; Visual Purple

Species: Human

Assay Type: Sandwich

Sensitivity: 0.58 ng/mL

Standard: 100 ng/mL

Detection range: 1.57-100 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Ophthalmology & Otorhinolaryngology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RHO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RHO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RHO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RHO in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: OPN2; RP4; Opsin 2; Rod Pigment; Retinitis Pigmentosa 4; Visual Purple

Species: Human

Assay Type: Sandwich

Sensitivity: 0.58 ng/mL

Standard: 100 ng/mL

Detection range: 1.57-100 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Ophthalmology & Otorhinolaryngology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RHO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RHO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RHO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RHO in the samples is then determined by comparing the OD of the samples to the standard curve.