ELK4637-96T, Mouse FATP5(Fatty Acid Transport Protein 5) ELISA Kit, 96T

Alternative Names: SLC27A5; ACSB; ACSVL6; FACVL3; VLACSR; BACS; VLCSH2; Fatty Acid Coenzyme A Ligase Very Long Chain 3; Solute Carrier Family 27 Member 5; Bile Acyl CoA Synthetase

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.054 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids.

Assay length: 3.5h

Research Area: Metabolic pathway;Cardiovascular biology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FATP5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FATP5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FATP5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FATP5 in the samples is then determined by comparing the OD of the samples to the standard curve.