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ELK4619-96T, Human CS(Citrate Synthase) ELISA Kit, 96T
2.963,10 RON
Human CS(Citrate Synthase) ELISA Kit
SKU
ELK4619-96T
Alternative Names: Citrate (Si)-synthase; mitochondrial
Species: Human
Assay Type: Sandwich
Sensitivity: 2.39 ng/mL
Standard: 400 ng/mL
Detection range: 6.25-400 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;Developmental science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CS in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Citrate (Si)-synthase; mitochondrial Species: Human Assay Type: Sandwich Sensitivity: 2.39 ng/mL Standard: 400 ng/mL Detection range: 6.25-400 ng/mL Sample type: Serum, plasma, tissue homogenates and other biological fluids. Assay length: 3.5h Research Area: Enzyme & Kinase;Metabolic pathway;Developmental science; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CS in the samples is then determined by comparing the OD of the samples to the standard curve. |
