ELK4541-96T, Human ACACa(Acetyl Coenzyme A Carboxylase Alpha) ELISA Kit, 96T

ELK4541-96T, Human ACACa(Acetyl Coenzyme A Carboxylase Alpha) ELISA Kit, 96T

ELK4542-96T, Human PSMa7(Proteasome Subunit Alpha Type 7) ELISA Kit, 96T

ELK4542-96T, Human PSMa7(Proteasome Subunit Alpha Type 7) ELISA Kit, 96T

ELK4542-48T, Human PSMa7(Proteasome Subunit Alpha Type 7) ELISA Kit, 48T

2.142,00 RON

Human PSMa7(Proteasome Subunit Alpha Type 7) ELISA Kit

SKU
ELK4542-48T

Alternative Names: C6; HSPC; RC6-1; XAPC7

Species: Human

Assay Type: Sandwich

Sensitivity: 0.059 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PSMa7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PSMa7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PSMa7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PSMa7 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: C6; HSPC; RC6-1; XAPC7

Species: Human

Assay Type: Sandwich

Sensitivity: 0.059 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PSMa7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PSMa7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PSMa7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PSMa7 in the samples is then determined by comparing the OD of the samples to the standard curve.