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ELK4533-48T, Rat PKCe(Protein Kinase C Epsilon) ELISA Kit, 48T
2.142,00 RON
Rat PKCe(Protein Kinase C Epsilon) ELISA Kit
SKU
ELK4533-48T
Alternative Names: PKC-E; PRKCE; PRKC-E; NPKC-Epsilon
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.58 ng/mL
Standard: 100 ng/mL
Detection range: 1.57-100 ng/mL
Sample type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Enzyme & Kinase;Apoptosis;Endocrinology;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PKCe. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PKCe. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PKCe, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PKCe in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.800,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: PKC-E; PRKCE; PRKC-E; NPKC-Epsilon Species: Rat Assay Type: Sandwich Sensitivity: 0.58 ng/mL Standard: 100 ng/mL Detection range: 1.57-100 ng/mL Sample type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 3.5h Research Area: Signal transduction;Enzyme & Kinase;Apoptosis;Endocrinology;Cardiovascular biology; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PKCe. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PKCe. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PKCe, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PKCe in the samples is then determined by comparing the OD of the samples to the standard curve. |
