ELK4484-96T, Human MHCDQb1(Major Histocompatibility Complex Class II DQ Beta 1) ELISA Kit, 96T

ELK4484-96T, Human MHCDQb1(Major Histocompatibility Complex Class II DQ Beta 1) ELISA Kit, 96T

ELK4485-96T, Human PTPN1(Protein Tyrosine Phosphatase, Non Receptor Type 1) ELISA Kit, 96T

ELK4485-96T, Human PTPN1(Protein Tyrosine Phosphatase, Non Receptor Type 1) ELISA Kit, 96T

ELK4485-48T, Human PTPN1(Protein Tyrosine Phosphatase, Non Receptor Type 1) ELISA Kit, 48T

2.142,00 RON

Human PTPN1(Protein Tyrosine Phosphatase, Non Receptor Type 1) ELISA Kit

SKU
ELK4485-48T

Alternative Names: PTP1B; PTP1-B; Protein-tyrosine phosphatase 1B

Species: Human

Assay Type: Sandwich

Sensitivity: 0.054 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PTPN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PTPN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PTPN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PTPN1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: PTP1B; PTP1-B; Protein-tyrosine phosphatase 1B

Species: Human

Assay Type: Sandwich

Sensitivity: 0.054 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PTPN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PTPN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PTPN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PTPN1 in the samples is then determined by comparing the OD of the samples to the standard curve.