ELK4445-48T, Human BRAF(B-Raf Proto Oncogene Serine/Threonine Protein Kinase) ELISA Kit, 48T

Alternative Names: B-raf 1; BRAF1; RAFB1; V-Raf Murine Sarcoma Viral Oncogene Homolog B1

Species: Human

Assay Type: Sandwich

Sensitivity: 0.118 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BRAF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BRAF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BRAF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BRAF in the samples is then determined by comparing the OD of the samples to the standard curve.