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ELK4331-96T, Human VCP(Valosin Containing Protein) ELISA Kit, 96T
2.963,10 RON
Human VCP(Valosin Containing Protein) ELISA Kit
SKU
ELK4331-96T
Alternative Names: IBMPFD; p97; TERA; Transitional Endoplasmic Reticulum ATPase; 15S Mg(2+)-ATPase p97 subunit
Species: Human
Assay Type: Sandwich
Sensitivity: 0.116 ng/mL
Standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VCP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VCP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human VCP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VCP in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: IBMPFD; p97; TERA; Transitional Endoplasmic Reticulum ATPase; 15S Mg(2+)-ATPase p97 subunit Species: Human Assay Type: Sandwich Sensitivity: 0.116 ng/mL Standard: 20 ng/mL Detection range: 0.32-20 ng/mL Sample type: tissue homogenates, cell lysates and other biological fluids Assay length: 3.5h Research Area: Neuro science; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VCP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VCP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human VCP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VCP in the samples is then determined by comparing the OD of the samples to the standard curve. |
