ELK4323-96T, Human FKBP1A(FK506 Binding Protein 1A) ELISA Kit, 96T

Alternative Names: FKBP-12; FKBP1; FKBP12C; PKC12; PKCI2; Calstabin-1; Rotamase; 12 kDa FK506-binding protein; Peptidyl-prolyl cis-trans isomerase FKBP1A; Immunophilin FKBP12

Species: Human

Assay Type: Sandwich

Sensitivity: 13.2 pg/mL

Standard: 2000 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Immune molecule;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FKBP1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FKBP1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FKBP1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FKBP1A in the samples is then determined by comparing the OD of the samples to the standard curve.