ELK4293-96T, Human ATP7b(ATPase, Cu++ Transporting Beta Polypeptide) ELISA Kit, 96T

Alternative Names: PWD; WC1; WD; WND; Wilson Disease Protein; Copper pump 2; Wilson disease-associated protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.056 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Signal transduction;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ATP7b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ATP7b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ATP7b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ATP7b in the samples is then determined by comparing the OD of the samples to the standard curve.