ELK4275-48T, Human PAR1(Protease Activated Receptor 1) ELISA Kit, 48T

ELK4275-48T, Human PAR1(Protease Activated Receptor 1) ELISA Kit, 48T

ELK4276-48T, Human PPM1A(Protein Phosphatase, Mg2+/Mn2+ Dependent 1A) ELISA Kit, 48T

ELK4276-48T, Human PPM1A(Protein Phosphatase, Mg2+/Mn2+ Dependent 1A) ELISA Kit, 48T

ELK4275-96T, Human PAR1(Protease Activated Receptor 1) ELISA Kit, 96T

2.963,10 RON

Human PAR1(Protease Activated Receptor 1) ELISA Kit

SKU
ELK4275-96T

Alternative Names: FIIR; F2r; CF2R; HTR; PAR1; TR; Coagulation Factor II Receptor; Thrombin Receptor

Species: Human

Assay Type: Sandwich

Sensitivity: 0.055 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Cardiovascular biology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PAR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PAR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PAR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PAR1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: FIIR; F2r; CF2R; HTR; PAR1; TR; Coagulation Factor II Receptor; Thrombin Receptor

Species: Human

Assay Type: Sandwich

Sensitivity: 0.055 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Cardiovascular biology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PAR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PAR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PAR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PAR1 in the samples is then determined by comparing the OD of the samples to the standard curve.