ELK4195-96T, Human ABI1(Abl Interactor 1) ELISA Kit, 96T

ELK4195-96T, Human ABI1(Abl Interactor 1) ELISA Kit, 96T

ELK4196-96T, Human EOMES(Eomesodermin) ELISA Kit, 96T

ELK4196-96T, Human EOMES(Eomesodermin) ELISA Kit, 96T

ELK4196-48T, Human EOMES(Eomesodermin) ELISA Kit, 48T

2.142,00 RON

Human EOMES(Eomesodermin) ELISA Kit

SKU
ELK4196-48T

Alternative Names: TBR2; T-Box Brain 2

Species: Human

Assay Type: Sandwich

Sensitivity: 0.115 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Signal transduction;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EOMES. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EOMES. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EOMES, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EOMES in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: TBR2; T-Box Brain 2

Species: Human

Assay Type: Sandwich

Sensitivity: 0.115 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Signal transduction;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EOMES. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EOMES. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EOMES, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EOMES in the samples is then determined by comparing the OD of the samples to the standard curve.