ELK4139-96T, Human Nesp1(Nesprin 1) ELISA Kit, 96T

Alternative Names: SYNE1; C6orf98; 8B; MYNE1; CPG2; SCAR8; ARCA1; Enaptin; Myocyte nuclear envelope protein 1; Nuclear envelope spectrin repeat protein 1; Synaptic nuclear envelope protein 1

Species: Human

Assay Type: Sandwich

Sensitivity: 0.105 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Immune molecule;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Nesp1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Nesp1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Nesp1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Nesp1 in the samples is then determined by comparing the OD of the samples to the standard curve.