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ELK4109-96T, Human FZD1(Frizzled Homolog 1) ELISA Kit, 96T
2.963,10 RON
Human FZD1(Frizzled Homolog 1) ELISA Kit
SKU
ELK4109-96T
Alternative Names: FzE1; Wnt Receptor
Species: Human
Assay Type: Sandwich
Sensitivity: 0.053 ng/mL
Standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Tumor immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FZD1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FZD1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FZD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FZD1 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: FzE1; Wnt Receptor Species: Human Assay Type: Sandwich Sensitivity: 0.053 ng/mL Standard: 10 ng/mL Detection range: 0.16-10 ng/mL Sample type: Tissue homogenates and other biological fluids. Assay length: 3.5h Research Area: Tumor immunity; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FZD1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FZD1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FZD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FZD1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
