ELK4071-96T, Human HBb(Hemoglobin Beta) ELISA Kit, 96T

Alternative Names: HB-B; HBD; CD113t-C; Beta Globin; Spinorphin

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 2.94 pg/mL

Standard: 500 pg/mL

Detection range: 7.82-500 pg/mL

Sample type: serum, plasma and erythrocyte lysates

Assay length: 2h

Research Area: Hematology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HBb protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HBb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HBb in the samples is then determined by comparing the OD of the samples to the standard curve.