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ELK3981-96T, Human OAS2(2',5'-Oligoadenylate Synthetase 2) ELISA Kit, 96T

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ELK3982-48T, Human OAS3(2',5'-Oligoadenylate Synthetase 3) ELISA Kit, 48T

2.142,00 RON

Human OAS3(2',5'-Oligoadenylate Synthetase 3) ELISA Kit

SKU
ELK3982-48T

Alternative Names: p100OAS

Species: Human

Assay Type: Sandwich

Sensitivity: 0.062 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OAS3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OAS3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human OAS3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OAS3 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: p100OAS

Species: Human

Assay Type: Sandwich

Sensitivity: 0.062 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OAS3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OAS3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human OAS3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OAS3 in the samples is then determined by comparing the OD of the samples to the standard curve.