ELK3976-96T, Human GJb1(Gap Junction Protein Beta 1) ELISA Kit, 96T

Alternative Names: CMTX1; CX32; GJ-B1; Connexin 32; Charcot-Marie-Tooth Neuropathy,X-Linked; GAP junction 28 kDa liver protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.108 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Tumor immunity;Infection immunity;Neuro science;Bone metabolism;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GJb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GJb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GJb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GJb1 in the samples is then determined by comparing the OD of the samples to the standard curve.