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ELK3939-48T, Human COL13(Collagen Type XIII) ELISA Kit, 48T
2.142,00 RON
Human COL13(Collagen Type XIII) ELISA Kit
SKU
ELK3939-48T
Alternative Names: COL13A1; COL13-A1; Collagen Alpha-1(XIII)chain
Species: Human
Assay Type: Sandwich
Sensitivity: 0.055 ng/mL
Standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Metabolic pathway;Developmental science;Nutrition metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COL13. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL13. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COL13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COL13 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.800,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: COL13A1; COL13-A1; Collagen Alpha-1(XIII)chain Species: Human Assay Type: Sandwich Sensitivity: 0.055 ng/mL Standard: 10 ng/mL Detection range: 0.16-10 ng/mL Sample type: tissue homogenates, cell lysates and other biological fluids Assay length: 3.5h Research Area: Metabolic pathway;Developmental science;Nutrition metabolism; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COL13. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL13. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COL13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COL13 in the samples is then determined by comparing the OD of the samples to the standard curve. |
