ELK3890-96T, Human DPP6(Dipeptidyl Peptidase 6) ELISA Kit, 96T

Alternative Names: DPPX; Dipeptidylpeptidase 6; Dipeptidyl aminopeptidase-like protein 6; Dipeptidyl aminopeptidase-related protein; Dipeptidyl peptidase IV-like protein

Species: Human

Assay Type: Sandwich

Sensitivity: 1.08 ng/mL

Standard: 200 ng/mL

Detection range: 3.13-200 ng/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DPP6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DPP6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DPP6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DPP6 in the samples is then determined by comparing the OD of the samples to the standard curve.