ELK3887-48T, Human HNRPK(Heterogeneous Nuclear Ribonucleoprotein K) ELISA Kit, 48T

ELK3887-48T, Human HNRPK(Heterogeneous Nuclear Ribonucleoprotein K) ELISA Kit, 48T

ELK3888-48T, Human KRT14(Keratin 14) ELISA Kit, 48T

ELK3888-48T, Human KRT14(Keratin 14) ELISA Kit, 48T

ELK3887-96T, Human HNRPK(Heterogeneous Nuclear Ribonucleoprotein K) ELISA Kit, 96T

2.963,10 RON

Human HNRPK(Heterogeneous Nuclear Ribonucleoprotein K) ELISA Kit

SKU
ELK3887-96T

Alternative Names: hnRNP-K; CSBP; HNRNPK; TUNP; Transformation Upregulated Nuclear Protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.122 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Signal transduction;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HNRPK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HNRPK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HNRPK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HNRPK in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: hnRNP-K; CSBP; HNRNPK; TUNP; Transformation Upregulated Nuclear Protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.122 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Signal transduction;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HNRPK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HNRPK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HNRPK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HNRPK in the samples is then determined by comparing the OD of the samples to the standard curve.