ELK3839-96T, Human FcgR3A(Fc Fragment Of IgG Low Affinity IIIa Receptor) ELISA Kit, 96T

Alternative Names: CD16a; CD16-A; FCGR3-A; FCG3; FcgRIII; IGFR3; NA; Neutrophil Antigen; Low affinity immunoglobulin gamma Fc region receptor III-A

Species: Human

Assay Type: Sandwich

Sensitivity: 50 ng/mL

Standard: 2000 ng/mL

Detection range: 31.25-2000 ng/mL

Sample type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: CD & Adhesion molecule;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FcgR3A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FcgR3A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FcgR3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FcgR3A in the samples is then determined by comparing the OD of the samples to the standard curve.