ELK3774-48T, Rat DKK1(Dickkopf Related Protein 1) ELISA Kit, 48T

Alternative Names: SK; Dickkopf Homolog 1(Xenopus Laevis)

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.27 ng/mL

Standard: 50 ng/mL

Detection range: 0.79-50 ng/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Signal transduction;Tumor immunity;Hematology;Developmental science;Bone metabolism;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat DKK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat DKK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat DKK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat DKK1 in the samples is then determined by comparing the OD of the samples to the standard curve.