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ELK3767-96T, Human THBS4(Thrombospondin 4) ELISA Kit, 96T
2.963,10 RON
Human THBS4(Thrombospondin 4) ELISA Kit
SKU
ELK3767-96T
Alternative Names: TSP4
Species: Human
Assay Type: Sandwich
Sensitivity: 0.065 ng/mL
Standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Plasma
Assay length: 3.5h
Research Area: Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human THBS4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human THBS4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human THBS4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human THBS4 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: TSP4 Species: Human Assay Type: Sandwich Sensitivity: 0.065 ng/mL Standard: 10 ng/mL Detection range: 0.16-10 ng/mL Sample type: Plasma Assay length: 3.5h Research Area: Hematology; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human THBS4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human THBS4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human THBS4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human THBS4 in the samples is then determined by comparing the OD of the samples to the standard curve. |
