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ELK3764-48T, Mouse AGRP(Agouti Related Protein) ELISA Kit, 48T
2.142,00 RON
Mouse AGRP(Agouti Related Protein) ELISA Kit
SKU
ELK3764-48T
Alternative Names: AGRT; ASIP2; ART; Agouti-Related Peptide
Species: Mouse
Assay Type: Sandwich
Sensitivity: 11.7 pg/mL
Standard: 2000 pg/mL
Detection range: 31.25-2000 pg/mL
Sample type: serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Metabolic pathway;Neuro science;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse AGRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse AGRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse AGRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse AGRP in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.800,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: AGRT; ASIP2; ART; Agouti-Related Peptide Species: Mouse Assay Type: Sandwich Sensitivity: 11.7 pg/mL Standard: 2000 pg/mL Detection range: 31.25-2000 pg/mL Sample type: serum, plasma, tissue homogenates, cell culture supernates and other biological fluids Assay length: 3.5h Research Area: Signal transduction;Metabolic pathway;Neuro science;Hormone metabolism; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse AGRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse AGRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse AGRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse AGRP in the samples is then determined by comparing the OD of the samples to the standard curve. |
