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ELK3714-96T, Mouse IL5Ra(Interleukin 5 Receptor Alpha) ELISA Kit, 96T
2.963,10 RON
Mouse IL5Ra(Interleukin 5 Receptor Alpha) ELISA Kit
SKU
ELK3714-96T
Alternative Names: CDw125; CD125; IL5R-A; IL5-RA; HSIL5R3; IL5R; IL5R-A;IL-5RA
Species: Mouse
Assay Type: Sandwich
Sensitivity: 28.5 pg/mL
Standard: 4000 pg/mL
Detection range: 62.5-4000 pg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Signal transduction;CD & Adhesion molecule;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL5Ra. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL5Ra. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL5Ra, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL5Ra in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: CDw125; CD125; IL5R-A; IL5-RA; HSIL5R3; IL5R; IL5R-A;IL-5RA Species: Mouse Assay Type: Sandwich Sensitivity: 28.5 pg/mL Standard: 4000 pg/mL Detection range: 62.5-4000 pg/mL Sample type: Serum, plasma and other biological fluids. Assay length: 3.5h Research Area: Signal transduction;CD & Adhesion molecule;Infection immunity; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL5Ra. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL5Ra. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL5Ra, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL5Ra in the samples is then determined by comparing the OD of the samples to the standard curve. |
