ELK3676-96T, Human GAD1(Glutamate Decarboxylase 1, Brain) ELISA Kit, 96T

Alternative Names: GAD67; SCP; 67 kDa glutamic acid decarboxylase; Glutamate decarboxylase 67 kDa isoform

Species: Human

Assay Type: Sandwich

Sensitivity: 0.061 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids.

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GAD1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GAD1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GAD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GAD1 in the samples is then determined by comparing the OD of the samples to the standard curve.