ELK3675-48T, Human HCCS(Holocytochrome C Synthase) ELISA Kit, 48T

ELK3675-48T, Human HCCS(Holocytochrome C Synthase) ELISA Kit, 48T

ELK3676-48T, Human GAD1(Glutamate Decarboxylase 1, Brain) ELISA Kit, 48T

ELK3676-48T, Human GAD1(Glutamate Decarboxylase 1, Brain) ELISA Kit, 48T

ELK3675-96T, Human HCCS(Holocytochrome C Synthase) ELISA Kit, 96T

2.963,10 RON

Human HCCS(Holocytochrome C Synthase) ELISA Kit

SKU
ELK3675-96T

Alternative Names: CCHL; MCOPS7; Cytochrome C Heme-Lyase

Species: Human

Assay Type: Sandwich

Sensitivity: 0.059 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Infection immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HCCS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HCCS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HCCS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HCCS in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: CCHL; MCOPS7; Cytochrome C Heme-Lyase

Species: Human

Assay Type: Sandwich

Sensitivity: 0.059 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Infection immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HCCS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HCCS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HCCS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HCCS in the samples is then determined by comparing the OD of the samples to the standard curve.