ELK3624-96T, Human LOXL4(Lysyl Oxidase Like Protein 4) ELISA Kit, 96T

ELK3624-96T, Human LOXL4(Lysyl Oxidase Like Protein 4) ELISA Kit, 96T

ELK3625-96T, Human ESM1(Endothelial Cell Specific Molecule 1) ELISA Kit, 96T

ELK3625-96T, Human ESM1(Endothelial Cell Specific Molecule 1) ELISA Kit, 96T

ELK3625-48T, Human ESM1(Endothelial Cell Specific Molecule 1) ELISA Kit, 48T

2.142,00 RON

Human ESM1(Endothelial Cell Specific Molecule 1) ELISA Kit

SKU
ELK3625-48T

Alternative Names: Endocan

Species: Human

Assay Type: Sandwich

Sensitivity: 6.2 pg/mL

Standard: 1000 pg/mL

Detection range: 15.63-1000 pg/mL

Sample type: serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Metabolic pathway;Tumor immunity;Infection immunity;Cardiovascular biology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ESM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ESM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ESM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ESM1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: Endocan

Species: Human

Assay Type: Sandwich

Sensitivity: 6.2 pg/mL

Standard: 1000 pg/mL

Detection range: 15.63-1000 pg/mL

Sample type: serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Metabolic pathway;Tumor immunity;Infection immunity;Cardiovascular biology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ESM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ESM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ESM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ESM1 in the samples is then determined by comparing the OD of the samples to the standard curve.