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ELK3555-48T, Mouse MyD88(Myeloid Differentiation Factor 88) ELISA Kit, 48T
2.142,00 RON
Mouse MyD88(Myeloid Differentiation Factor 88) ELISA Kit
SKU
ELK3555-48T
Alternative Names: Myeloid Differentiation Primary Response Gene 88
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.061 ng/mL
Standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;CD & Adhesion molecule;Tumor immunity;Pulmonology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MyD88. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MyD88. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MyD88, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MyD88 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.800,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Myeloid Differentiation Primary Response Gene 88 Species: Mouse Assay Type: Sandwich Sensitivity: 0.061 ng/mL Standard: 10 ng/mL Detection range: 0.16-10 ng/mL Sample type: Tissue homogenates, cell lysates and other biological fluids Assay length: 3.5h Research Area: Signal transduction;CD & Adhesion molecule;Tumor immunity;Pulmonology; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MyD88. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MyD88. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MyD88, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MyD88 in the samples is then determined by comparing the OD of the samples to the standard curve. |
