ELK3549-96T, Human IFNa/bR2(Interferon Alpha/Beta Receptor 2) ELISA Kit, 96T

Alternative Names: IFNαR2; IFN-alpha-REC; IFN-R; IFNαBR; IFNαRB; Interferon(Alpha,Beta And Omega)Receptor Beta Chain; Interferon alpha binding protein; Type I interferon receptor 2

Species: Human

Assay Type: Sandwich

Sensitivity: 0.262 ng/mL

Standard: 40 ng/mL

Detection range: 0.63-40 ng/mL

Sample type: Serum, plasma, urine and other biological fluids.

Assay length: 3.5h

Research Area: Infection immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IFNa/bR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IFNa/bR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IFNa/bR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IFNa/bR2 in the samples is then determined by comparing the OD of the samples to the standard curve.