ELK3529-48T, Human MCM5(Minichromosome Maintenance Deficient 5) ELISA Kit, 48T

Alternative Names: CDC46; P1-CDC46; Cell Division Cycle 46; DNA replication licensing factor MCM5

Species: Human

Assay Type: Sandwich

Sensitivity: 0.061 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Developmental science;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MCM5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MCM5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MCM5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MCM5 in the samples is then determined by comparing the OD of the samples to the standard curve.