ELK3510-48T, Human CA6(Carbonic Anhydrase VI) ELISA Kit, 48T

Alternative Names: CA-VI; CAVI; GUSTIN; Carbonate dehydratase VI; Salivary carbonic anhydrase; Secreted carbonic anhydrase

Species: Human

Assay Type: Sandwich

Sensitivity: 0.131 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: Serum, plasma, saliva and other biological fluids

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CA6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CA6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CA6 in the samples is then determined by comparing the OD of the samples to the standard curve.