ELK3509-48T, Human NES(Nestin) ELISA Kit, 48T

Alternative Names: ESTM 46 ; FLJ 21841 ; FLJ21841 ; Intermediate filament protein ; Nbla00170 ; nes ; NEST

Species: Human

Assay Type: Sandwich

Sensitivity: 0.054 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Neuro science;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NES. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NES. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NES, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NES in the samples is then determined by comparing the OD of the samples to the standard curve.