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ELK3414-96T, Rat KLK9(Kallikrein 9) ELISA Kit, 96T
2.963,10 RON
Rat KLK9(Kallikrein 9) ELISA Kit
SKU
ELK3414-96T
Alternative Names: KLK-L3; Kallikrein-Related Peptidase 9; Kallikrein-like protein 3
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.052 ng/mL
Standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;Tumor immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat KLK9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat KLK9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat KLK9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat KLK9 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: KLK-L3; Kallikrein-Related Peptidase 9; Kallikrein-like protein 3 Species: Rat Assay Type: Sandwich Sensitivity: 0.052 ng/mL Standard: 10 ng/mL Detection range: 0.16-10 ng/mL Sample type: Serum, plasma, tissue homogenates and other biological fluids. Assay length: 3.5h Research Area: Enzyme & Kinase;Tumor immunity; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat KLK9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat KLK9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat KLK9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat KLK9 in the samples is then determined by comparing the OD of the samples to the standard curve. |
