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ELK3373-96T, Human a1BG(Alpha-1-B-Glycoprotein) ELISA Kit, 96T
2.963,10 RON
Human a1BG(Alpha-1-B-Glycoprotein) ELISA Kit
SKU
ELK3373-96T
Alternative Names: A1BG; Alpha-1B-glycoprotein; Alpha-1-B glycoprotein
Species: Human
Assay Type: Sandwich
Sensitivity: 0.147 ng/mL
Standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human a1BG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human a1BG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human a1BG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human a1BG in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: A1BG; Alpha-1B-glycoprotein; Alpha-1-B glycoprotein Species: Human Assay Type: Sandwich Sensitivity: 0.147 ng/mL Standard: 20 ng/mL Detection range: 0.32-20 ng/mL Sample type: Serum, plasma and other biological fluids Assay length: 3.5h Research Area: Immune molecule; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human a1BG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human a1BG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human a1BG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human a1BG in the samples is then determined by comparing the OD of the samples to the standard curve. |
