ELK3351-96T, Rat PHB(Prohibitin) ELISA Kit, 96T

Alternative Names: Epididymis luminal protein 215 ; Epididymis secretory sperm binding protein Li 54e ; HEL 215 ; HEL S 54e ; PHB1

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.121 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 3.5h

Research Area: Tumor immunity;Hormone metabolism;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PHB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PHB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PHB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PHB in the samples is then determined by comparing the OD of the samples to the standard curve.