ELK3318-48T, Human SPON1(Spondin 1) ELISA Kit, 48T

Alternative Names: VSGP; F-spondin; F-spondin,Extracellular Matrix Protein; Vascular smooth muscle cell growth-promoting factor

Species: Human

Assay Type: Sandwich

Sensitivity: 0.235 ng/mL

Standard: 40 ng/mL

Detection range: 0.63-40 ng/mL

Sample type: serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Signal transduction;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SPON1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SPON1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SPON1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SPON1 in the samples is then determined by comparing the OD of the samples to the standard curve.