ELK3294-48T, Mouse VS(Versican) ELISA Kit, 48T

Alternative Names: CSPG2; VCAN; ERVR; PG-M; WGN1; Chondroitin Sulfate Proteoglycan 2; Chondroitin sulfate proteoglycan core protein 2; Glial hyaluronate-binding; Large fibroblast proteoglycan

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.065 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Developmental science;Bone metabolism;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse VS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VS in the samples is then determined by comparing the OD of the samples to the standard curve.