ELK3255-48T, Human SIRT1(Sirtuin 1) ELISA Kit, 48T

ELK3255-48T, Human SIRT1(Sirtuin 1) ELISA Kit, 48T

ELK3256-48T, Mouse CNTN1(Contactin 1) ELISA Kit, 48T

ELK3256-48T, Mouse CNTN1(Contactin 1) ELISA Kit, 48T

ELK3255-96T, Human SIRT1(Sirtuin 1) ELISA Kit, 96T

2.963,10 RON

Human SIRT1(Sirtuin 1) ELISA Kit

SKU
ELK3255-96T

Alternative Names: SIR2L1; Silent Mating Type Information Regulation 2 Homolog 1; NAD-Dependent Deacetylase Sirtuin-1; SIR2-like protein 1; SirtT1 75 kDa fragment

Species: Human

Assay Type: Sandwich

Sensitivity: 0.29 ng/mL

Standard: 50 ng/mL

Detection range: 0.79-50 ng/mL

Sample type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Endocrinology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SIRT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SIRT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SIRT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SIRT1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: SIR2L1; Silent Mating Type Information Regulation 2 Homolog 1; NAD-Dependent Deacetylase Sirtuin-1; SIR2-like protein 1; SirtT1 75 kDa fragment

Species: Human

Assay Type: Sandwich

Sensitivity: 0.29 ng/mL

Standard: 50 ng/mL

Detection range: 0.79-50 ng/mL

Sample type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Endocrinology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SIRT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SIRT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SIRT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SIRT1 in the samples is then determined by comparing the OD of the samples to the standard curve.