ELK3240-48T, Human MUTYH(MutY Homolog) ELISA Kit, 48T

ELK3240-48T, Human MUTYH(MutY Homolog) ELISA Kit, 48T

ELK3241-48T, Rat APC(Activated Protein C) ELISA Kit, 48T

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ELK3240-96T, Human MUTYH(MutY Homolog) ELISA Kit, 96T

2.963,10 RON

Human MUTYH(MutY Homolog) ELISA Kit

SKU
ELK3240-96T

Alternative Names: MYH; A/G-specific adenine DNA glycosylase

Species: Human

Assay Type: Sandwich

Sensitivity: 0.057 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Tumor immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MUTYH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MUTYH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MUTYH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MUTYH in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: MYH; A/G-specific adenine DNA glycosylase

Species: Human

Assay Type: Sandwich

Sensitivity: 0.057 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Tumor immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MUTYH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MUTYH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MUTYH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MUTYH in the samples is then determined by comparing the OD of the samples to the standard curve.