ELK3219-48T, Human CPT1B(Carnitine Palmitoyltransferase 1B, Muscle) ELISA Kit, 48T

ELK3219-48T, Human CPT1B(Carnitine Palmitoyltransferase 1B, Muscle) ELISA Kit, 48T

ELK3220-48T, Human TSPAN1(Tetraspanin 1) ELISA Kit, 48T

ELK3220-48T, Human TSPAN1(Tetraspanin 1) ELISA Kit, 48T

ELK3219-96T, Human CPT1B(Carnitine Palmitoyltransferase 1B, Muscle) ELISA Kit, 96T

2.963,10 RON

Human CPT1B(Carnitine Palmitoyltransferase 1B, Muscle) ELISA Kit

SKU
ELK3219-96T

Alternative Names: CPT1-M; Carnitine palmitoyltransferase I-like protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.125 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CPT1B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CPT1B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CPT1B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CPT1B in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: CPT1-M; Carnitine palmitoyltransferase I-like protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.125 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CPT1B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CPT1B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CPT1B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CPT1B in the samples is then determined by comparing the OD of the samples to the standard curve.