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ELK3185-96T, Human ENPP2(Ectonucleotide Pyrophosphatase/Phosphodiesterase 2) ELISA Kit, 96T
2.963,10 RON
Human ENPP2(Ectonucleotide Pyrophosphatase/Phosphodiesterase 2) ELISA Kit
SKU
ELK3185-96T
Alternative Names: ATX; ATX-X; LysoPLD; NPP2; PD-IALPHA; PDNP2; Autotaxin; Extracellular lysophospholipase D
Species: Human
Assay Type: Sandwich
Sensitivity: 43.21 pg/mL
Standard: 6000 pg/mL
Detection range: 93.75-6000 pg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ENPP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ENPP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ENPP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ENPP2 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: ATX; ATX-X; LysoPLD; NPP2; PD-IALPHA; PDNP2; Autotaxin; Extracellular lysophospholipase D Species: Human Assay Type: Sandwich Sensitivity: 43.21 pg/mL Standard: 6000 pg/mL Detection range: 93.75-6000 pg/mL Sample type: Serum, plasma and other biological fluids. Assay length: 3.5h Research Area: Enzyme & Kinase; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ENPP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ENPP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ENPP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ENPP2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
