Limba
Romanian
Romanian
English|
ELK3134-96T, Human ICOS(Inducible T-Cell Co Stimulator) ELISA Kit, 96T
2.963,10 RON
Human ICOS(Inducible T-Cell Co Stimulator) ELISA Kit
SKU
ELK3134-96T
Alternative Names: CD278; AILIM; Inducible T-Cell Costimulator; Activation-Inducible Lymphocyte Immunomediatory Molecule
Species: Human
Assay Type: Sandwich
Sensitivity: 5.8 pg/mL
Standard: 1000 pg/mL
Detection range: 15.63-1000 pg/mL
Sample type: serum, plasma, urine, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Tumor immunity;Infection immunity;Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ICOS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ICOS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ICOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ICOS in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: CD278; AILIM; Inducible T-Cell Costimulator; Activation-Inducible Lymphocyte Immunomediatory Molecule Species: Human Assay Type: Sandwich Sensitivity: 5.8 pg/mL Standard: 1000 pg/mL Detection range: 15.63-1000 pg/mL Sample type: serum, plasma, urine, cell lysates and other biological fluids Assay length: 3.5h Research Area: Signal transduction;Tumor immunity;Infection immunity;Immune molecule; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ICOS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ICOS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ICOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ICOS in the samples is then determined by comparing the OD of the samples to the standard curve. |
