ELK3053-48T, Human PSPN(Persephin) ELISA Kit, 48T

ELK3053-48T, Human PSPN(Persephin) ELISA Kit, 48T

ELK3054-48T, Human ANKRD1(Ankyrin Repeat Domain Protein 1) ELISA Kit, 48T

ELK3054-48T, Human ANKRD1(Ankyrin Repeat Domain Protein 1) ELISA Kit, 48T

ELK3053-96T, Human PSPN(Persephin) ELISA Kit, 96T

2.963,10 RON

Human PSPN(Persephin) ELISA Kit

SKU
ELK3053-96T

Alternative Names: PSP

Species: Human

Assay Type: Sandwich

Sensitivity: 13.4 pg/mL

Standard: 2000 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 3.5h

Research Area: Cytokine;Infection immunity;Neuro science;Developmental science;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PSPN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PSPN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PSPN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PSPN in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: PSP

Species: Human

Assay Type: Sandwich

Sensitivity: 13.4 pg/mL

Standard: 2000 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 3.5h

Research Area: Cytokine;Infection immunity;Neuro science;Developmental science;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PSPN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PSPN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PSPN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PSPN in the samples is then determined by comparing the OD of the samples to the standard curve.