ELK3025-48T, Human sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit, 48T

Alternative Names: MAC; Membrane Attack Complex; C5b-9; Terminal Complement Complex C5b-9; C5b9

Species: Human

Assay Type: Sandwich

Sensitivity: 0.51 ng/mL

Standard: 80 ng/mL

Detection range: 1.25-80 ng/mL

Sample type: serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Signal transduction;Metabolic pathway;Apoptosis;Infection immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sC5b-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sC5b-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sC5b-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sC5b-9 in the samples is then determined by comparing the OD of the samples to the standard curve.